Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas | Knowledge Trend

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About the Work

Class: A Research Project

Field: Applied Microbiology

Author: Abasiama Akpan

Number of Pages: 71

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ABSTRACT

Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 C for 42 days. Five digesters were used for the experiment and each digester contained Oryza sativa husks and Musa paradisiaca in the ratio of 1:1 with cow dung as inoculum. Substrate in Digester X (Control) was not pretreated,  Digester A (Oryza sativa husks pretreated hydrothermally), B (Oryza sativa husks pretreated with cassava mill effluent), C (Substrates pretreated hydrothermally + acid fermentation with cassava mill effluent), whereas D contained Musa paradisiaca peels and Oryza sativa husks mixture subjected to combined pretreatment. During the digestion period, Digester X (control) generated the lowest cumulative biogas of 68mL gVS-1 while Digester C produced the highest biogas yield of 209mL gVS-1. Digestate obtained from the Digester C contained microbial load of 6.31Log10CFU g-1 while the control contained lowest microbial load of 5.23Log10CFU g-1. Methanogens of genera Methanosarcina, Methanocorpusculum, Methanobacterium, Methanobrevibacter and Methanothrix were isolated from the digestates. The Substrates contained total solids of 7.88, 7.49, 6.53, 6.87 and 6.02mg kg¹; and volatile solids of 7.07, 7.02, 6.36, 6.61 and 5.28mg kg¹, respectively. After digestion, digestates analyzed contained total solids of 5.21, 4.94, 5.01, 5.04, 5.2604mg kg¹; and volatile solids of 5.15, 4.81, 4.91, 4.49 and 5.12mg kg¹ for Digester X, A, B, C, and D respectively.. Therefore this experiment has proved that Oryza sativa husks and peels of Musa paradisiaca are potential substrates for biogas production.

Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas | Knowledge Trend

A Graphical Representation of the Cumulative Biogas Yield from the different digesters.





TABLE OF CONTENTS

TITLE PAGE

Inner page       -           -           -           -           -           -           -           -          -           i

Certification    -           -           -           -           -           -           -           -          -           ii

Dedication       -           -           -           -           -           -           -           -        -           iii

Acknowledgements    -           -           -           -           -           -           -       -           iv

Table of contents        -           -           -           -           -           -           -           -        v-vi

List of figures  -           -           -           -           -           -           -           -           -         ii

List of tables   -           -           -           -           -           -           -           -           -        viii

Abstract          -           -           -           -           -           -           -           -           -          ix


CHAPTER ONE

1.1       Introduction    -           -           -           -           -           -         -           -           1-4

1.2       Objective of study     -           -           -           -           -           -           -           5

CHAPTER TWO: LITERATURE REVIEW

2.1       Types of feedstock / substrates for biogas production           -           -           6-7

2.1.1    Agricultural wastes with manure co-digestion           -             - -   8-10

2.1.2    Energy crops with manure as substrate         -           -         -           -           10-11

2.1.3    Food wastes with manure as substrate           -           -           -           -         11

2.1.4    Industrial wastes and by-products as substrates        -           -           -          11-12

2.1.5    Manure as substrates for biogas production  -           -           -           -         12

2.2       Processes of anaerobic digestion and microorganisms involved       -         12-13

2.2.1    Hydrolysis      -           -           -           -           -           -           -           -        13

2.2.2    Acidogenesis   -           -           -           -           -           -           -           -          14

2.2.3    Acetogenesis   -           -           -           -           -           -           -           -          14-15


2.2.4    Methanogenesis         -           -           -           -           -                      -           15-16

2.2.5    Types of anaerobic digestion  -           -           -          -           -          -           18

2.2.6    Advantages of anaerobic digestion     -           -                -           -            19       

2.3       Factors affecting anaerobic digestion for biogas production -           -            19       

2.3.1    Nature or type of substrate/ sub-layer composition    -           -             -         19-21

2.3.2    Digester temperature  -           -           -           -           -           -           -           21

2.3.3    Hydraulic retention time (HRT)        -           -           -           -           -             22

2.3.4    pH       -           -           -           -           -           -           -           -           -         22-23

2.3.5    Working pressure in the fermenter     -           -           -           -           -           23-24

2.3.6    Digester design -         -           -           -           -           -           -           -           24

2.4       Methods of improving biogas production      -           -           -           -           25

2.4.1    Pretreatment    -           -           -           -           -           -           -           -           25-30

2.4.2    Co-digestion   -           -           -           -           -           -           -           -           31-32

2.4.3    Microbial bioaugmentation     -           -           -           -           -           -           32

2.4.4    Agitation of the reaction system         -           -           -           -           -           32-33  

2.5       Composition and utilization of biogas            -           -           -           -          33-34

2.6       Sources of the substrates and their chemical composition     -           -           - 34-37

CHAPTER THREE: MATERIALS AND METHODS

3.0       Equipment and supplies          -           -           -           -           -           -           -           38

3.1       Aseptic techniques and sterilization procedure          -           -           -           -           38

3.2       Sample collection and processing      -           -           -           -           -           -           39

3.3       Experimental design   -           -           -           -           -           -           -           -           39-41  

3.3.1    Digestion of substrate -           -           -           -           -           -           -           -           41

3.3.2    Measurement of biogas           -           -           -           -           -           -           -           41-43

3.4       Determination of total and volatile solids (TS and VS)         -           -           -           44       

3.5       Microbial analysis       -           -           -           -           -           -           -           -           44

3.5.1    Media preparation       -           -           -           -           -           -           -           -           44

3.5.2    Isolation of anaerobic bacteria            -           -           -           -           -           -           45

3.5.3    Characterization and identification of bacteria isolates          -           -           -           45-48

CHAPTER FOUR: RESULT

4.0       Results -           -           -           -           -           -           -           -           -           -           49-54


CHAPTER FIVE: DISCUSSION AND CONCLUSION

5.1       Discussionand conclusion      -           -           -           -           -           -           -           -55-56  

5.2       Conclusion      -           -           -           -           -           -           -           -           -           56       


REFERENCES        -           -           -           -           -           -           -           -           -           57-65


LIST OF FIGURES



Figure Title Page

2.1 Stages of anaerobic digestion and associated microbial groups - - 17

3.1 Diagram of the experimental design - - - - - - -      43

4.1 Cumulative biogas yield - - - - - - - -        -       -    51

4.2 Total solid content of the substrates and digestates - - - - - 52

4.3 Total Volatile solid content of the substrates and digestates - - - 52

4.4 Counts of anaerobic microorganisms associated with the biogas production in the digestate - - - - - - - - - 53



LIST OF TABLES


Table Title Page

2.1 Some constituents of selected lignocellulosic materials - - - -    8

2.2 Comparison between dry and wet digestion - - - - - -    -    18

2.3 Methods of pretreatment of lignocellulosic materials - - - -    -    30

2.4 Percentage composition of biogas - - - - - - -    -    -    -  34

3.1 Composition of basal medium used for selective enrichment and growth of some methanogenic species isolated from the substrates - - - - 48

4.1 Morphological and biochemical characteristics of methanogenic bacteria species 

Isolated from the digestate - - - - - - - - 54



Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas | Knowledge Trend


Cite This Work

APA
Abasiama, A. (2019). Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas. Knowledge Trend. Retrieved from https://www.knowledgetrend.com/2022/07/codigestion-of-oryza-sativa-husks-and.html.

MLA 8th
Abasiama, Abasiama, and Akpan John "Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas". Knowledge Trend. Knowledge Trend. 17 Nov. 2019, https://www.knowledgetrend.com/2022/07/codigestion-of-oryza-sativa-husks-and.html. Accessed 25 Jun. 2022 (Todays Date).

MLA7
Abasiama, Abasiama, and Akpan John . "Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas". Knowledgetrend, 17 Nov. 2019. Web. 22 Jun. 2022. https://www.knowledgetrend.com/2022/07/codigestion-of-oryza-sativa-husks-and.html

Chicago
Abasiama, Abasiama and John, Akpan . "Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas" Knowledg Trend (2019). Accessed July 25, 2022. https://www.knowledgetrend.com/2022/07/codigestion-of-oryza-sativa-husks-and.html


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