@article{ran2013,
  title={Genome engineering using the CRISPR-Cas9 system},
  author={F. Ann Ran and Patrick D. Hsu and Jason Wright and Vineeta Agarwala and David Scott and Feng Zhang},
  year={2013},
  journal={Nature Protocols},
  doi={10.1038/nprot.2013.143},
  url={https://doi.org/10.1038/nprot.2013.143}
}